Coding

Part:BBa_K3386003:Design

Designed by: Daria Pająk, Gustav Aberg   Group: iGEM20_Aalto-Helsinki   (2020-10-22)


EGFP is a reporter protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The sequence was codon optimized to lower the complexity and enable de novo synthesis.



Source

Aequorea victoria


References

1. Te-Tuan Yang, Linzhao Cheng, Steven R. Kain, Optimized Codon Usage and Chromophore Mutations Provide Enhanced Sensitivity with the Green Fluorescent Protein, Nucleic Acids Research, Volume 24, Issue 22, 1 November 1996, Pages 4592–4593, https://doi.org/10.1093/nar/24.22.4592

2. Site-specific incorporation of unnatural amino acids as probes for protein conformational changes. Peeler JC, Mehl RA. Methods Mol Biol. 2012;794:125-34. doi: 10.1007/978-1-61779-331-8_8. 10.1007/978-1-61779-331-8_8 PubMed 21956560